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Gel Permeation Chromatography
Non-aqueous GPC column
| High speed & high resolution |
COSMOSIL ® non-aqueous GPC columns 5GPC-60, 5GPC-100 and 5GPC-300 can provide high resolution, high-speed analysis. GPC packing materials are 5µm diameter particles of totally porous spherical silica gel. Without swelling they display excellent stability against pressure.
For GPC, all samples are eluted within t0. The t0 value for a COSMOSIL ® 5GPC 4.6mmI.D.x250mm column is about three minutes at a flow rate of 1.0ml/min; meaning that the analysis is completed within about three minutes. COSMOSIL ® 5GPC column therefore require less solvent. This is important, especially when expensive solvents such as 1,1,1,3,3,3-hexafluoro-2-propanol are used. In each molecular weight range, the GPC columns provide linear correlation between log MW and the elution volume. The large pore volume results in high peak capacity. Higher efficiencies are obtained with multiple columns. Coupled columns (GPC-60 and GPC-100, or GPC-100 and GPC-300) are frequently employed to meet the wide molecular weight range of samples.
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| Column characteristics |
Type |
Particle size |
Pore size |
MW range |
| COSMOSIL ® 5GPC-60 |
5µm |
60Å |
300-15,000 |
| COSMOSIL ® 5GPC-100 |
5µm |
100Å |
1000-50,000 |
| COSMOSIL ® 5GPC-300 |
5µm |
300Å |
17,000-300,000 |
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| Recommended solvents and optimal temperature |
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| - Solvents |
Saturated hydrocarbons and the solvents specified below can be used with the GPC column. The use of solvents and mobile phases containing water should be avoided in order to prevent deterioration of column efficiency.
Solvents from the halogen series may corrode the stainless steel tubing and fittings of the HPLC system. After using these solvents, wash the column with solvent not containing halogen ions. Column washing and changing of mobile phase should be conducted with low flow rate. Flow rate of less than 0.5ml/min for 4.6mmI.D. columns and less than 1.5ml/min for 10mmI.D. columns are recommended.
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| -Temperature |
Run the separation at a column temperature of 50°C lower than the boiling point of the mobile phase in order to prevent bubble formation.
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| Organic solvents for non-aqueous GPC columns |
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Physical property of the solvent |
Toxicity |
SOLVENT |
Boiling point
°C |
Viscosity
(20°C)cp. |
Refractive index(20°C) |
Flash Point°C |
LD 50 mg/kg |
L(ct) 50 ppm |
1,1,1,3,3,3- Hexafluoro -2- propanol |
59 |
- |
1.2700 |
- |
- |
- |
Tetrahydrofuran |
66 |
0.51(25°C) |
1.4070 |
14 |
hazardous |
200 |
1,2,4- Trichlorobenzene |
213 |
1.89(25°C) |
1.5717 |
99 |
hazardous |
75 |
o- Dichlorobenzene |
180 |
1.26 |
1.5515 |
66 |
500 |
50 |
Toluene |
110.6 |
0.59 |
1.4961 |
4 |
hazardous |
6000-200 |
N,N- Dimethylformamide |
153 |
0.90 |
1.4280 |
58 |
hazardous |
100-10 |
Dichloromethane |
40.1 |
0.30 |
1.4242 |
- |
3000 |
500 |
1,2- Dichloroethane |
84 |
0.84 |
1.4443 |
13 |
770 |
50 |
N- Methylpyrrolidone |
202 |
1.65 |
1.47 |
95.4 |
hazardous |
- |
m- Cresol |
202.8 |
20.8 |
1.544 |
94 |
hazardous |
5 |
Benzene |
80.1 |
0.652 |
1.5011 |
27 |
7000 |
25 |
Dimethyl Sulioxide |
189 |
- |
1.477 |
95 |
hazardous |
- |
Tetrachloroethylene |
121 |
- |
1.505 |
- |
hazardous |
100 |
o- Chlorophenol |
175.5 |
4.11 |
1.5473 |
- |
670 |
dangerous |
CarbonTetrachloride |
76.8 |
0.969 |
1.4607 |
- |
hazardous |
10 |
Trifluoroethanol |
73.6 |
1.20
(38°C) |
1.291 |
40.6 |
240 |
4600 |
Chloroform |
61.7 |
0.58 |
1.446 |
- |
hazardous |
50 |
Acetone |
56.2 |
0.316 |
1.359 |
-17.8 |
4-8 |
500 |
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| Catalog
# |
Cosmosil 5GPC-60 Column |
Price ($U.S.) |
- |
| 093-7830 |
5GPC-60 - Packed Column, 4.6 x 250mm |
$680.00 |
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| 093-7826 |
5GPC-60 - Packed Column, 10 x 250mm |
$1,630.00 |
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| Catalog
# |
Cosmosil 5GPC-100 Column |
Price ($U.S.) |
- |
| 093-9732 |
5GPC-100 - Packed Column, 4.6 x 250mm |
$680.00 |
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| 093-9744 |
5GPC-100 - Packed Column, 10 x 250mm |
$1,470.00 |
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| Catalog
# |
Cosmosil 5GPC-300 Column |
Price ($U.S.) |
- |
| 093-9736 |
5GPC-300 - Packed Column, 4.6 x 250mm |
$680.00 |
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| 093-9752 |
5GPC-300 - Packed Column, 10 x 250mm |
$1,630.00 |
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