|
Cosmosil
AR-300 Series
Reversed-phase
HPLC is the preferred separation method for the analysis of
proteins and peptides, due to the superior resolution, high
theoretical plate number, good reproducibility, ease of use
and favorable cost performance.
In general, trifluoroacetic acid (TFA) is more often used
as an acid mobile phase, in the analysis of proteins and peptides.
However, reversed-phase HPLC using conventional silica gel
shows some degradation over time in these very acidic conditions.
The COSMOSIL AR-300 series packed columns have succeeded
in improving acid resistance due largely to the development
of a new synthesis method. It can be used for a longer time,
while providing sharp peak shape even in the concentrations
of 0.1% of trifluoroacetic acid.
The
COSMOSIL AR-300 series packed column show superior stability
in the following decomposition test under the strong
acidic conditions. |
Capacity
factor: toluene, Mobile phase :60% methanol |
|
Three
alkyl and one aromatic type stationary phases are available
in the COSMOSIL AR-300 series packed column.
Please consult the table and applications below for
selection guidance. |
Alkyl
type: 5C18-AR-300, 5C8-AR-300,
5C4-AR-300
Aromatic type: 5Ph-AR-300 |
MATERIAL
CHARACTERISTICS |
|
5C18-AR-300 |
5C8-AR-300 |
5C4-AR-300 |
5Ph-AR-300 |
Silica
gel |
high
purity spherical porous silica |
Average
particle size |
approx.
300Å |
Specific
surface area |
approx.
150m²/g |
Stationary
phase |
octadecyl
group |
octyl
group |
butyl
group |
phenyl
group |
End
capping treatment |
near-perfect
treatment |
Carbon
content |
approx.
12% |
approx.
7% |
approx.
6% |
approx.
7% |
Semi-prep
separation proteins |
The
sample loading capacity for protein or polypeptide separations
is proportional to the cross section of column inside
diameter. Using a short column, one can load a relatively
large quantity of sample due to the absorption and concentration
of sample inside the column. Below examples are shown
for the separation using COSMOSIL 5C18-AR-300, with
4.6mmI.D. x 150mm column, 10mmI.D. x 150mm and 10mmI.D.
x 250mm columns.
Identical separation patterns are achieved by adjusting
proportionally the flow rate and the sample injection
volume to the cross section of the column. There is
little difference between 10mmI.D. x 150mm column and
10mmI.D. x 250mm column. Thus, semi-prep separations
may be achieved using a shorter column. |
|
| Catalog
# |
Cosmosil 5C18 -AR-300 Column |
Price ($U.S.) |
- |
| 093-7921 |
5C18 -AR-300 - Analytical Column, 2.0 x 150 mm |
$410.00 |
 |
| 093-7911 |
5C18 -AR-300 - Analytical Column, 4.6 x 50 mm |
$325.00 |
|
| 093-7912 |
5C18 -AR-300 - Analytical Column, 4.6 x 100 mm |
$450.00 |
|
| 093-7913 |
5C18 -AR-300 - Analytical Column, 4.6 x 150 mm |
$510.00 |
|
| 093-7914 |
5C18 -AR-300 - Analytical Column, 4.6 x 250 mm |
$625.00 |
|
| 093-7915 |
5C18 -AR-300 - Semi-preparative Column, 6 x 150 mm |
$510.00 |
|
| 093-7916 |
5C18 -AR-300 - Semi-preparative Column, 6x 250 mm |
$800.00 |
|
| 093-7917 |
5C18 -AR-300 - Preparative Column, 10 x 150 mm |
$950.00 |
|
| 093-7918 |
5C18 -AR-300 - Preparative Column, 10 x 250 mm |
$1,200.00 |
|
| 093-7919 |
5C18 -AR-300 - Preparative Column, 20 x 150 mm |
$1,870.00 |
|
| 093-7920 |
5C18 -AR-300 - Preparative Column, 20 x 250 mm |
$2,390.00 |
|
| 093-7910 |
5C18 -AR-300 - Analytical Guard Column, 4.6 x 10.0 mm |
$240.00 |
|
| 093-7965 |
5C18 -AR-300 - Semi-preparative Guard Column, 10 x 20 mm |
$420.00 |
|
| Catalog
# |
Cosmosil 5C8-AR-300 Column |
Price ($U.S.) |
- |
| 093-7951 |
5C8-AR-300 - Analytical Column, 4.6.x 50 |
$320.00 |
|
| 093-7952 |
5C8-AR-300 - Analytical Column, 4.6 x 100 mm |
$420.00 |
|
| 093-7953 |
5C8-AR-300 - Analytical Column, 4.6 x 150 mm |
$480.00 |
|
| 093-7954 |
5C8-AR-300 - Analytical Column, 4.6 x 250 mm |
$600.00 |
|
| 093-7950 |
5C8-AR-300 - Analytical Guard Column, 4.6 x 10.0 mm |
$240.00 |
|
| Catalog
# |
Cosmosil 5C4-AR-300 Column |
Price ($U.S.) |
- |
| 093-7956 |
5C4-AR-300 - Analytical Column, 4.6 x 50 mm |
$320.00 |
|
| 093-7957 |
5C4-AR-300 - Analytical Column, 4.6 x 100 mm |
$420.00 |
|
| 096-7958 |
5C4-AR-300 - Analytical Column, 4.6 x150 mm |
$480.00 |
|
| 093-7959 |
5C4-AR-300 - Analytical Column, 4.6 x 250 mm |
$600.00 |
|
| 093-8047 |
5C4-AR-300 - Semi-preparative Column, 10 x 250 mm |
$1,160.00 |
|
| 093-8048 |
5C4-AR-300 - Preparative Column, 20 x 250 mm |
$2,300.00 |
|
| 093-7955 |
5C4-AR-300 - Analytical Guard Column, 4.6 x 10 mm |
$240.00 |
|
| Catalog
# |
Cosmosil 5Ph-AR-300 Column |
Price ($U.S.) |
- |
| 093-7961 |
5Ph-AR-300 - Analytical Column, 4.6.x 50 |
$320.00 |
|
| 093-7962 |
5Ph-AR-300 - Analytical Column, 4.6 x 100 mm |
$420.00 |
|
| 093-7963 |
5Ph-AR-300 - Analytical Column, 4.6 x 150 mm |
$480.00 |
|
| 093-7964 |
5Ph-AR-300 - Analytical Column, 4.6 x 250 mm |
$580.00 |
|
| 093-7960 |
5Ph-AR-300 - Analytical Guard Column, 4.6 x 10.0 mm |
$240.00 |
|
| 093-7961 |
5Ph-AR-300 - Analytical Column, 4.6.x 50 mm |
$320.00 |
|
| 093-7962 |
5Ph-AR-300 - Analytical Column, 4.6 x 100 mm |
$420.00 |
|
| 093-7963 |
5Ph-AR-300 - Analytical Column, 4.6 x 150 mm |
$480.00 |
|
| 093-7964 |
5Ph-AR-300 - Analytical Column, 4.6 x 250 mm |
$580.00 |
|
| 093-7960 |
5Ph-AR-300 - Analytical Guard Column, 4.6 x 10.0 mm |
$240.00 |
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